Fig. 3

Overexpression of FABP5 promotes fatty acid synthesis in KGN cells. A Bar plot showing the expression of FABP5 in KGN cells after transfection with the PCS2-myc-FABP5 or empty vector plasmids for 48 h, as determined via qRT‒PCR. B, C Immunoblotting plot (B) and bar plot of statistical analysis (C) showing the expression of FABP5 in KGN cells transfected with PCS2-myc-FABP5 or empty vector plasmids for 48 h. D Nile red staining of KGN cells after 48 h of FABP5 overexpression. The red signal indicates the formation of lipid droplets. All the samples were also stained with DAPI. Scale bar, 100 μm. E Statistical analysis of the number of lipid droplets. Five fields of view were randomly selected. F Bar plot showing the ACSL1, GPAM1, LPIN1 and DGAT2 mRNA expression in KGN cells after 48 h of FABP5 expression. A two-tailed unpaired t test was used for all the statistical analyses in this section. * P < 0.05, ** P < 0.01, ***P < 0.001. The groups transfected with the empty vector control or pCS2-myc-FABP5 plasmids were referred to as the EV-ctrl and myc-FABP5 groups, respectively