Fig. 4

Knockdown of FABP5 inhibits fatty acid synthesis in KGN cells. A Bar plot showing the expression of FABP5 in KGN cells after transfection with FABP5 or negative control siRNAs for 48 h. B, C Immunoblot plot (B) and bar plot of the statistical analysis (C) showing the expression of FABP5 in KGN cells transfected with FABP5 or negative control siRNAs for 48 h. D Nile red staining diagram of KGN cells after transfection with FABP5 siRNA for 48 h. The red signal indicates the formation of lipid droplets. All the samples were also stained with DAPI. Scale bar, 100 μm. E Statistical analysis of the number of lipid droplets. Five fields of view were randomly selected. F Bar plot showing ACSL1, GPAM1, LPIN1 and DGAT2 mRNA expression in KGN cells after transfection with FABP5 siRNA for 48 days. A two-tailed unpaired t test was used for all the statistical analyses in this section. * P < 0.05, ** P < 0.01, ***P < 0.001. The groups transfected with the negative control siRNA or with FABP5 siRNA were referred to as siNC and siFABP5, respectively