Fig. 5

FABP5 facilitates KGN cell proliferation. A Immunoblotting plot showing the expression of FABP5 and the cell proliferation marker PCNA in KGN cells after 48 h of FABP5 overexpression. B A line chart showing the proliferation of KGN cells after 48 h of FABP5 overexpression, as determined by CCK8 assays. C Immunofluorescence staining of Ki67 in KGN cells after 48 h of FABP5 overexpression. All the samples were also stained with DAPI. Scale bar, 100 μm. D Statistical analysis of the Ki67-positive cells. Five fields of view were randomly selected. E Immunoblotting plot showing the expression of FABP5 and PCNA in KGN cells after transfection with FABP5 or negative control siRNAs for 48 h. F Line chart showing the proliferation of KGN cells after transfection with FABP5 or negative control siRNAs for 48 h, as determined by CCK8 assays. G Immunofluorescence staining of Ki67 in KGN cells after transfection with FABP5 or negative control siRNAs for 48 h. All samples were also stained with DAPI. Scale bar, 100 μm. H Statistical analysis of the Ki67-positive cells. Five fields of view were randomly selected. A two-tailed unpaired t test was used for all the statistical analyses. * P < 0.05, ** P < 0.01, ***P < 0.001