Fig. 7

FABP5 accelerates fatty acid synthesis in KGN cells by activating PI3K-AKT signaling (A, C) Nile red staining of KGN cells treated with 10 μM LY294002 or SC79 for 48 h after 48 h of FABP5 overexpression or knockdown. The red signal indicates the formation of lipid droplets. All the samples were also stained with DAPI. Scale bar, 100 μm. B, D Statistical analysis of the number of lipid droplets. Five fields of view were randomly selected. E, G Bar plot showing ACSL1, GPAM1, LPIN1 and DGAT2 mRNA expression in KGN cells treated with 10 μM LY294002 or SC79 for 48 h after FABP5 overexpression or knockdown. A two-tailed unpaired t test was used for all the statistical analyses. *P < 0.05, **P < 0.01, ***P < 0.001.