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Table 1 qRT-PCR primers sequences

From: High levels of fatty acid-binding protein 5 excessively enhances fatty acid synthesis and proliferation of granulosa cells in polycystic ovary syndrome

Gene

Forward Primers (5′-3′)

Reverse Primer (5′-3')

Homo sapiens FABP5

AGGAGTGGGAATAGCTTTGCG

GCTGAACCAATGCACCATCT

Homo sapiens ACSL1

AAGACAGATGGGAGGAGACCC

GCACGTACTGTCGGAAGTCA

Homo sapiens GPAM

GAAGCTGGAGCTGCTAGGG

CCACACTCACCCCATTCCTC

Homo sapiens LPIN1

CCAGCCTGCTGAGAACTAGA

GTGGAAAGGGGAGCATTGGA

Homo sapiens DGAT2

GCTGGGGTCTAGGCTGTTTC

TAGGCGGCTATGAGGGTCTT

Homo sapiens 18S rRNA

CGGCTACCACATCCAAGGAA

CTGGAATTACCGCGGCT

Mus musculus Fabp5

ACCGAGAGCACAGTGAAGAC

ACCGAGAGCACAGTGAAGAC

Mus musculus 18 s rRNA

ATGGCCGTTCTTAGTTGGTG

CGGACATCTAAGGGCATCAC

  1. Data were analyzed by using the 2−ΔΔCt relative quantitative method with human or mouse 18 s rRNA as an internal control and A two-tailed unpaired t-test was used for statistical analysis
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Journal of Ovarian Research

ISSN: 1757-2215

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