Fig. 1
From: Structural basis for antibody recognition of the proximal MUC16 ectodomain
Humanization of 4H11. (a) Sequence alignment of; 4H11, human frame template and humanized h4H11 heavy and light chains, mVH and mVL, heavy chain and light chain of 4H11 mouse IgG1, hVHt, the framework template of human heavy chain (4dtg), hVLt, the framework template of human light chain (3O2d). hVH1 and 2, hVL1 and 2, and the humanized heavy chain and light chain of 4H11. Yellow highlighted sequences are CDRs. Humanized amino acids indicated in red letters. (b) Structural overlay of 4H11 and humanized antibody structures. The structures of 4H11 and humanized antibody (4H11, hH1L1, hH1L2, hH2L1, hH2L2) were simulated by ABodyBuilder. 4H11 heavy (sky blue) and light (pink) chains. hH1L1 heavy (TV blue) and light (dirty violet) chains. hH1L2 heavy (marine) and light (violet purple) chains. hH2L1 heavy (deep blue) and light (purple) chains. hH2L2 heavy (density) and light (deep purple) chains. (c) Binding kinetics of 4H11 and humanized h4H11 antibodies by ForteBio Octet analysis