Fig. 4

Molecular validations of TGFBI functions. A, B TGFBI KO confirmed by WB in SKOV-3 cell line and Caov-3 cell line. C, D Analysis of the expression changes of EMT markers (CDH1, CDH2 and MMP-2) in TGFBI KO cell lines at mRNA level, values were normalized to GAPDH. E, F Analysis of the expression changes of EMT markers (CDH1, CDH2 and MMP-2) in TGFBI KO cell lines at protein level. KO knock out, WB western blot, MMP-2 matrix metallopeptidase 2, GAPDH glyceraldehyde-3-phosphate dehydrogenase. *p < 0.05, **p < 0.01, ***p < 0.001