Fig. 1

Cisplatin promoted the KGN cells apoptosis, decreased the expression of FTO, and activated the Hippo/YAP signaling pathway, in a dose dependent manner. (A-B) Flow cytometry assays were performed to determine the effect of cisplatin on KGN cells apoptosis. And a quantitative analysis of the rate of apoptosis was performed. (C-M) Western blot assay was applied to detect the effect of cisplatin on the protein expression level of apoptosis related protein, including Bcl-2, BAX, and cleaved-caspase-3, FTO and the key molecular of Hippo/YAP signaling pathway. β-actin was used as a loading control. (N-O) Immunofluorescence assay was performed to detect the effect of cisplatin on the expression and location of YAP1. (scale bar: 100 μm). Student’s t-test. The results were showed mean ± SEM from three independent experiments. (α = 0.05, *P < 0.05, **P < 0.01, ***P < 0.001)