Fig. 3

Downregulation of FTO promoted KGN cells apoptosis and activated the Hippo/YAP signaling pathway. (A) Western blotting was used to detect the effects of si-FTO on the expression of FTO, Bcl − 2, BAX, MST, p-MST, LATS, p-LATS1, YAP1, and p - YAP1. β-actin was used as a loading control. (B-K) Quantitative comparison of expression levels of FTO, Bcl-2, BAX, p-MST1/MST1, p-LATS1/LATS1, p-YAP1/YAP1 in different groups. (L-N) The mRNA expression level of CTGF, CYR61, and ANKRD1 were determined by qRT-PCR. GAPDH was used as a loading control. Student’s t-test. The results were showed mean ± SEM from three independent experiments. (α = 0.05, *P < 0.05, **P < 0.01, ***P < 0.001). (O-P) Immunofluorescence assay was performed to determine the effect of si-FTO on the expression and location of YAP1. (scale bar: 100 μm)