Fig. 4

Overexpression of FTO promoted cisplatin-induced injured KGN cells proliferation and decreased their apoptosis via Hippo/YAP signaling pathway, while VP reversed it. (A) Western blotting was used to detect the effects of FTO on the expression of Bcl-2, BAX, cleaved-caspase-3, PCNA, MST, p-MST, LATS1, p-LATS1, YAP1, and p-YAP1in injured KGN cells. β-actin was used as a loading control. (B-K) Quantitative comparison of expression levels Bcl-2, BAX, cleaved-caspase-3, PCNA, MST, p-MST, LATS1, p-LATS1, YAP1, and p-YAP1 in different groups. (L) CCK-8 assay was performed to measure the rate the KGN cells with different treatments. (M-N) Flow cytometry assays were performed to determine the apoptosis rate in different groups, and the quantitative comparison was included. One-way ANOVA followed by Tukey’s multiple comparison test. The results were showed mean ± SEM from 3 independent experiments. (α = 0.05, *P < 0.05, **P < 0.01, ***P < 0.001). (O-P) Immunofluorescence assay was performed to determine the expression and location of YAP1 in different groups. (scale bar: 100 μm)