Fig. 8
Experimental validation of IRF6. A The mRNA expression of IRF6 in OC tissues and adjacent normal tissues was measured by qRT-PCR (B) Immunohistochemistry of IRF6 based on the OC tissues and adjacent normal tissues. C The mRNA expression of IRF6 in SKOV3 cells was detected via qRT-PCR after transfection with siRNA targeting IRF6 for 48 h. D CCK8 experiment results of SKOV3 cells after cell transfection. E The scratch assay and statistical analysis of SKOV3 cells for the group original and si-IRF6. F The effect of IRF6 on invasion of SKOV3 cells was investigated by transwell invasion assay and statistical analysis. Data are mean ± SD from three independent experiments for D. The scales bar to indicate 50 μm for B. (**means P < 0.01; *** means P < 0.001, **** means P < 0.0001, N = 3, Student’s t-test, Error bars are ± SEM)