Skip to main content
Fig. 6 | Journal of Ovarian Research

Fig. 6

From: Corpus luteum presence in the bovine ovary increase intrafollicular progesterone concentration: consequences in follicular cells gene expression and follicular fluid small extracellular vesicles miRNA contents

Fig. 6

Characterization of small extracellular vesicles (sEVs) from follicular fluid (FF). (A). Size and (B) concentration of FF sEVs analyzed (n=6) by nanotracking analysis equipment. The black line between the error bars represents the group means and the error bars represent the standard error of the means. (C) Distribution of size and particles/Ml. (D) Western blotting analysis of Alix and Tomm20 in FF sEVs and follicular cells (FCs). The images demonstrated Alix presence, an accessory protein of the ESCRT complex, in sEVs and FCs. Tomm20, a translocase protein of the outer mitochondrial membrane complex, was not detected in sEVs, only in FCs. Western blot images were cropped and the entire membrane image can be found in the supplementary figure S1. (E) Transmission electron microscopy of FF sEVs isolated from follicular fluid, demonstrating the cup-shaped phenotype of the nanoparticles (black arrows)

Back to article page

Journal of Ovarian Research

ISSN: 1757-2215

Contact us