Fig. 1

Cellular localization of QSOX2 protein in the mouse ovarian tissue. A Indirect immunofluorescent stating showed the presence of mQSOX2 (in red) in the granulosa cells of follicles of different stages, including primary, secondary, and antral follicles. However, no mQSOX2 was detected in the corpus luteum. B Magnified images showed two distinct mQSOX2 cellular localizations. Based on the hematoxylin–eosin stain, we showed that the mQSOX2 identified was located at the follicle-supporting cells rather than within the oocyte. The oocyte orientation, referred to as QSOX2, is located in a straight line between the oocyte and the granulosa cell. The random orientation, referred to as QSOX2 located randomly in the granulosa cells and is not in a straight line between the oocyte and the granulosa cell