Fig. 5

RNAseq analysis revealed ovarian genes and signaling pathways affected by Qsox2 deletion. A The principal component analysis (PCA) presents the two distinct group distributions of the control and KO ovaries with a stress value of 1.0*10^–5. B Volcano plot of differentially expressed genes (DEGs) in the ovaries of Qsox2 KO mice. A total of 1821 differentially expressed genes (DEGs). 633 up- and 1188 down-regulated genes are indicated as red and green dots, respectively (fold change ≥ 2 and P-value < 0.05). C Selected signaling pathways and its associated heatmap showed the relative expression patterns of the 164 DEGs (54 up- and 110 down-regulated genes) between control and Qsox2 KO groups. The color scale of the heatmap ranges from green (down-regulation) to red (up-regulation)