Fig. 5
TGF-β1/smads pathway is essential for CDCA5-mediated invasion and migration in OC. A Heatmap showing differentially expressed genes among SKOV3/NC and SKOV3/sh3 cells. B Volcano plot shows differentially expressed genes between SKOV3/NC and SKOV3/sh3 cells. C The biological process with the top 20 enrichments is shown. D The top 20 functionally enriched KEGG pathways were shown in a scatter plot. E The signal transduction proteins in SKOV3 cells were detected by WB. F WB analysis explored the expression of TGF-β1,smad2/3, p-smad2, and p-smad3 with SB431542 (TGF-β1 inhibitor, 10 µM) in HEY cells. G The signal transduction proteins in A2780 cells were detected by WB. H Transwell assays were performed to investigate the invasion and migration of CDCA5 in HEY cells treated with or without SB431542. (*means CDCA5 group compared with vector, #means CDCA5 group compared with or without SB431542)