Table 2 The elution condition of the mobile phase (A: water; B: acetonitrile)
Time/min | Flow rate(μL/min) | Phase A% (water) | Phase B%(Acetonitrile) |
|---|---|---|---|
0 | 40 | 95 | 5 |
3.5 | 40 | 85 | 15 |
6 | 40 | 70 | 30 |
6.5 | 40 | 70 | 30 |
12 | 40 | 30 | 70 |
12.5 | 40 | 30 | 70 |
18 | 40 | 0 | 100 |
22 | 40 | 0 | 100 |
25 | 40 | 0 | 100 |
26 | 40 | 95 | 5 |
30 | 40 | 95 | 5 |