Fig. 3

MDHB attenuates TBHP induced KGN cells apoptosis. A, B TUNEL staining was performed to detected the effect of MDHB on TBHP induced GCs apoptosis. Representative immunofluorescence images of TUNEL (green) and nuclei labeled with DAPI. C-F Immunoblots showing the expression of Casp-3, C-Casp-3, Casp7, C-Casp-7, Casp-9 and C-Casp-9 in KGN cells treated with TBHP or MDHB (10, 20µM). Quantification of the protein levels relative to β-actin. All data were presented as the Means ± SD, *P < 0.05, **P < 0.01