Figure 2

Representative Western blots and mean levels (± S.E.M) of (A) Akt, (B) p-Akt, (C) Erk and (D) p-Erk in granulosa cells (n = 4) treated with control medium, FSH (0.33 ng/ml), IGF (10 ng/ml) or FSH+IGF in combination in vitro. Bars with no common superscript are different (P < 0.05). The units represent the intensity of bands after background subtraction and are relative to white (value 0) and black (value 256). The blots each show a single band for Akt and p-Akt at about 60 kDa and each show a double band for Erk and p-Erk at about 44 and 42 kDa.