Figure 5

A, Haptotactic migration toward collagen I. P, EV, and ST6 cells were serum starved for 48 hours. Cells were then seeded in serum-free media into the upper wells of Boyden chambers lined with 8.0 μm PET membranes coated on the underside with a collagen I. The lower chambers contained conditioned NIH3T3 media as a chemoattractant. Cells were allowed to migrate for 14 hours, and cell migration was quantified using the vendor's protocol. B, Invasion of OV4 cells through Matrigel-coated transwells. P, EV, and ST6 cells were serum starved for 48 hours, and then seeded into the upper wells of Boyden chambers lined with Matrigel-coated 8.0 μm PET membranes. The lower chambers contained condition NIH3T3 media as a chemoattractant. Cells were allowed to invade for 48 hours and invasion was quantified using the vendor's protocol. Data represent means and SEMs of three independent experiments run in triplicate. * denotes P < 0.01, evaluated by ANOVA.