Figure 3
From: Aberrant STYK1 expression in ovarian cancer tissues and cell lines
Estradiol and G-1 induce STYK1 RNA expression. Ovarian tumor cell lines were treated with vehicle, 5 × 10-8 M estradiol, 1 × 10-8 M BSA-conjugated estradiol (E2B), and 1 × 10-8 M G-1 for 4-18 h(T4, T8, T18). cDNA equivalent to 25 ng/μL of RNA was generated and analyzed by real-time RT-PCR. Relative values were normalized to α-tubulin and values were compared to the vehicle. Values are the mean of 3 independent experiments. (A) STYK1 induction in estradiol-treated ovarian tumor cells relative to untreated cells for various intervals; (B) STYK1 induction in E2-, E2B- and G1-treated cells relative to estradiol-induced STYK1 expression following 18 hours. * and ** indicate values that are significantly different compared to the vehicle (p < 0.01 and p < 0.001, respectively).