Figure 4
Differentiation status and miRNA biosynthesis gene expression in differentiated NTera2 and 2102Ep cells. The expression of markers of pluripotency (Oct4 and Nanog) and of endoderm (Afp), ectoderm (Ncam1) and mesoderm (Eno3) differentiation is shown. In each case, data represents changes in expression in the differentiate state compared to undifferentiated state. In NTera2 cells, differentiation status is confirmed by decreases in expression of Oct4 and Nanog and increases in expression of Afp, Ncam1 and Eno3. 2102Ep cells alter expression of these genes less than two-fold, confirming nullipotency.