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Figure 3 | Journal of Ovarian Research

Figure 3

From: Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs

Figure 3

Effect of H89, SB203580 or U0126 on expression of Has2 (A), Tnfaip6 (B) or Ptgs2 (C) mRNA. For reference, the 0 h COC value was set as 1 and the data presented as the fold strength. Values are mean +/- SEM of 3 replicates. *: The significant differences were observed as compared with that in COCs cultured with FSH and LH for 10 h. **: The significant differences were observed as compared with that in COCs cultured with FSH, LH, H89 and EGF for 10 h. ***: The significant differences were observed as compared with that in COCs cultured with FSH, LH, SB230580 and EGF for 10 h. The respective value of among Has2, Tnfaip6 and Ptgs2 mRNA were normalized according to those of β-actin mRNA to evaluate arbitrary units of the relative abundance of the targets. FSH(-): COCs were cultured without FSH and LH for 10 h; FSH(+): COCs were cultured with FSH and LH for 10 h; EGF(-): COCs were cultured without EGF for 10 h; EGF(+): COCs were cultured with EGF for 10 h; +H89: COCs were cultured with H89 for 10 h; +SB: COCs were cultured with SB203580 for 10 h; +U0126: COCs were cultured with U0126 for 10 h

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Journal of Ovarian Research

ISSN: 1757-2215

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