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Table 1 List of primers employed for RT-PCR

From: Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs

Gene Forward Primer Reverse Primer Anneling temprature (X) Cycle (Y)
β-actin 5'-CTA CAA TGA GCT GCG TGT GG-3' 5'-TAG CTC TTC TCC AGG GAG GA-3' 58 31
Areg 5'-CAC CAG AAC AAA AAG GTT CTG TC-3' 5'-AAG TCC ATG AAG ACT CAC ACC AT-3' 58 35
Ereg 5'AAG ACA ATC CAC GTG TGG CTC AAG-3' 5'-CGA TTT TTG TAC CAT CTG CAG AAA-3' 58 35
Tace/Adam17 5'-GAC ATG AAT GGC AAA TGT GAG AAA C-3' 5'-AGT CTG TGC TGG GGT CTT CCT GGA-3' 58 34
Has2 5'-GAA TTA CCC AGT CCT GGC TT-3' 5'-GGA TAA ACT GGT AGC CAA CA-3' 54 35
Tnfaip6 5'-TCA TAA CTC CAT ATG GCT TGA AC-3' 5'-TCT TCG TAC TCA TTT GGG AAG CC-3' 54 32
Ptgs2 5'-CTG CCG TGT CGC TCT GCA CTG-3' 5'-TCA TAA CTC CAT ATG GCT TGA AC-3' 58 35