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Table 1 List of primers employed for RT-PCR

From: Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs

Gene

Forward Primer

Reverse Primer

Anneling temprature (X)

Cycle (Y)

β-actin

5'-CTA CAA TGA GCT GCG TGT GG-3'

5'-TAG CTC TTC TCC AGG GAG GA-3'

58

31

Areg

5'-CAC CAG AAC AAA AAG GTT CTG TC-3'

5'-AAG TCC ATG AAG ACT CAC ACC AT-3'

58

35

Ereg

5'AAG ACA ATC CAC GTG TGG CTC AAG-3'

5'-CGA TTT TTG TAC CAT CTG CAG AAA-3'

58

35

Tace/Adam17

5'-GAC ATG AAT GGC AAA TGT GAG AAA C-3'

5'-AGT CTG TGC TGG GGT CTT CCT GGA-3'

58

34

Has2

5'-GAA TTA CCC AGT CCT GGC TT-3'

5'-GGA TAA ACT GGT AGC CAA CA-3'

54

35

Tnfaip6

5'-TCA TAA CTC CAT ATG GCT TGA AC-3'

5'-TCT TCG TAC TCA TTT GGG AAG CC-3'

54

32

Ptgs2

5'-CTG CCG TGT CGC TCT GCA CTG-3'

5'-TCA TAA CTC CAT ATG GCT TGA AC-3'

58

35