Figure 2

Percent change in the number of cisplatin-induced apoptotic OvCa cells. OVCAR-3 and SKOV-3 cells were cultured for 24 hours with 5 μg/ml of cisplatin alone or with 0 or 100 ng/ml CCL25 plus 1 μg/ml of anti-human CCR9 or isotype and untreated cell were used as controls. Cells were harvested and stained with annexin V and propidium iodide. Dual flowcytometric analysis of Annexin V-FITC and propidium iodide (PI) staining. Living cell populations are clustered in the Q3 quadrant; cells in early apoptosis are in the Q4 quadrant; late apoptotic/necrotic cells are in the Q2 quadrant.