Figure 2

Expression of PGD2-receptors in the mouse adult ovary. DP2 protein expression was detected in granulosa cells of primary, secondary and preantral follicles (A), of antral (B) and preovulatory (C) follicles of wild type adult ovary, using immunofluorescence staining with an anti-DP2 antibody (in red) whereas FOXL2 (A) or AMH (B,C) (in green) were used to delineate granulosa cells. Right panels are the merge between DP2 and FOXL2 or AMH stainings. Dotted lines delineate granulosa (GC) and theca (TC) cells. Scale bars = 50 μm. (D), Control immunofluorescence experiment using an anti-DP2 antibody with the Hoescht dye (HST) labeling nuclei. Dotted lines delineate granulosa (GC) and theca (TC) cells within a follicle. Scale bars = 25 μm. (E), Expression levels of PGD2 receptors Dp1 and Dp2 mRNAs by real time RT-PCR. Dp2 was expressed at high levels in ovaries from adult cycling mice (n = 4) whereas Dp1 transcripts were undetectable. The values of three repeats were averaged and normalized to Gapdh expression.