Figure 1

Immunohistochemistry. Representative microscopic images of OVISE cell line after exposure to 10 μg/ml CDDP for 24 h. γH2AX foci in a nuclear are stained green and red, respectively. An increase in green dots, indicating elevation of γH2AX, can be seen after exposure to CDDP. Thus, DNA damage is visually recognizable in each nucleus. Apoptotic bodies (insert) are distinguishable by the entire cell being intensely positive for γH2AX.