Figure 4

CD133 mRNA and protein expression in ovarian (OVCAR-8 and IGROV-1) and endometrial (Ishikawa) cancer cell lines before and after treatment with 5-aza-2`-deoxycytidine (DAC). A: Reverse transcription-polymerase chain reaction (RT-PCR) analysis of the CD133 gene in three cell lines with or without 5-aza-2‵-deoxycytidine (DAC). B: Representative results of real-time PCR analysis of CD133 expression in three cell lines with or without 5-aza-2‵-deoxycytidine (DAC). C: Immunoblot analysis of CD133 expression in three cell lines with or without 5-aza-2‵-deoxycytidine (DAC). D: Fluorescence-activated cell sorting analysis of CD133+ cells in three cell lines with or without 5-aza-2‵-deoxycytidine (DAC). * Results are representative of three independent experiments.