Figure 5

CD133 mRNA and protein expression in ovarian (OVCAR-8 and IGROV-1) and endometrial (Ishikawa) cancer cell lines before and after treatment with Trichostatin A (TSA). A: Reverse transcription-polymerase chain reaction (RT-PCR) analysis of the CD133 gene in three cell lines with or without Trichostatin A (TSA). B: Representative results of real-time PCR analysis of CD133 expression in three cell lines with or without Trichostatin A (TSA). C: Immunoblot analysis of CD133 expression in three cell lines with or without Trichostatin A (TSA). D: Fluorescence-activated cell sorting analysis of CD133+ cells in three cell lines with or without Trichostatin A (TSA). * Results are representative of three independent experiments.