Figure 8
Immuno-staining on control and PMSG treated ovarian sections. A-C: Immunostaining with anti-PCNA antibody. Note minimal PCNA staining in control OSE. Increased staining is observed after PMSG treatment in both the OSE cells and the oocytes of PF (B-&C). Inset B’ is magnified image of the PF located in the OSE. At places, granulosa cells were also positive for PCNA (arrowhead). D-F: Immunostaining with anti-OCT-4 antibody in control (D) and 7D PMSG treated (E&F) ovarian sections. OCT-4 is localized in the ooplasm in control, while in 7D PMSD treated, positive staining was observed in ooplasm as well as in the nucleus of few oocytes (arrowhead) in PFs. G: Immunostaining with anti-human VASA antibody that cross-reacts with mouse MVH. MVH is distinctly localized in the ooplasm of PF and at places was also observed in a ‘germ cell nest’ (G’, arrow). At places some oocytes of primordial follicles in cohorts appeared connected without intervening granulosa cell (arrowhead) H-J: SCP-3 is localized in PF oocytes present in close vicinity of multilayer OSE. Bar: 20μm.