Figure 4

A. Expression of cell adhesion molecules on mesothelial cells (MCs). MCs were exposed to IL-1β (20 ng/ml) for 30 minutes, and expression of a panel of adhesion molecules was assessed by flow cytometry. Untreated control was set as 100%. Results are presented as median values ± median absolute deviation in relation to control. Data are representative of five experiments. * p < 0.01. B. Inhibition of MFOC3 adhesion to mesothelial cells by 1, 10 or 100 ug/ml anti-β1 integrin * p < 0.05. Control (C) experiment was performed in the presence of 100 ug/ml mouse IgG. C. Summary of the effects of anti-IL-1β (10 ug/ml) and β1 integrin (10 ug/ml) on the binding to mesothelial cells of FOC3 and MFOC3, and FOC3 with recombinant IL-1β (20 ng/ml). Open bars represent FOC3; shaded bars, MFOC3; solid bars, FOC3 with recombinant IL-1β. Data are expressed as the mean ± SD of three separate experiments.