Figure 3
GPER, ERα and ERβ gene expression in ovarian cancer cell lines GPER, ERα, and ERβ mRNAs were normalized to corresponding β-actin mRNA in seven ovarian cancer cell lines (analyzed in 4-6 wells). Cell lines are arranged in order of declining GPER mRNA level. All cell lines expressed GPER mRNA and ERβ mRNA with highest levels in TOV21G and TOV112D. ERα mRNA was not detected in TOV21G, TOV112D, OVCAR3, ES2, and in insignificant amounts in SKOV-3.