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Figure 2 | Journal of Ovarian Research

Figure 2

From: Insulin and insulin-like growth factor signaling increases proliferation and hyperplasia of the ovarian surface epithelium and decreases follicular integrity through upregulation of the PI3-kinase pathway

Figure 2

Insulin and IGF-I increase OSE proliferation in a dose- and time-dependent manner. Ovarian organoids were cultured for 7d with increasing concentrations of insulin (A) or IGF-I (B) and analyzed for CK8 and BrdU expression. The percentage of OSE with BrdU incorporation was determined by dividing the number of cells positive for CK8 and BrdU by the total number of CK8-positive cells. C, Organoids were cultured for 1, 3, 7, or 14d in basal media (black line), media with 5 μg/ml insulin (blue line), or 5 μg/ml IGF-I (red line) and assessed for the percentage of OSE with BrdU incorporation as described above. All images are 400X. Data shown represent SEM. * indicates P<0.05.

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Journal of Ovarian Research

ISSN: 1757-2215

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