Figure 5

ALDH1 (+)- related miRNAs are associated with chemoresistance in ovarian cancer cells and tumor tissues. A. A comparison of miRNA expression in SKOV3 and chemoresistant SKpac cells. miRNAs-23b, -27b, -346, and −503 were significantly upregulated in SKpac cells compared with SKOV3 parent cells. B. Expression of ALDH1 (+)-related miRNAs by qRT-PCR in chemosensitive and chemoresistant ovarian cancer tissues. Relative expression levels were normalized to those of normal epithelial cells. Expression levels of miRNAs-23b, -27b, and −424 were significantly upregulated in the chemoresistant group compared with those in the chemosensitive group. Representative experiments repeated three times (*p < 0.05). C. High expression of miR-503 significantly correlated (p = 0.033) with advanced clinical stage (stage III and IV). D. Upregulation of miR-27a was associated with distant metastasis (p = 0.046).