Table 2 Primer sequence of stemness-related genes
From: MicroRNAs overexpressed in ovarian ALDH1-positive cells are associated with chemoresistance
Gene | Primer sequence |
|---|---|
Notch3 | F: 5′- TCT TGC TGC TGG TCA TTC TC-3′ |
R: 5′-TGC CTC ATC CTC TTC AGT TG-3′ | |
Oct4 | F: 5′-GGA AAG GCT TCC CCC TCA GGG AAA GG-3′ |
R: 5′-AAG AAC ATG TGT AAG CTG CGG CCC-3′ | |
NAC1 | F: 5′-CCA GAC ACT GCA GAT GGA GA-3′ |
R: 5′-AAG CTG AGG ATC TGC TGG AA-3′ | |
c-Kit | F: 5′-GGC ATC ACG GTG ACT TCA AT-3′ |
R: 5′-GGT TTG GGG AAT GCT TCA TA-3′ | |
BMI1 | F: 5′-TTA CCT GGA GAC CAG CAA GT-3′ |
R: 5′-CAT TAG AGC CAT TGG CAG CA′3′ | |
Nanog | F: 5′-AGA AGG CCT CAG CAC CTA C-3′ |
R: 5′-GGC CTG ATT GTT CCA GGA TT-3′ | |
Sox-2 | F: 5′-GCG CGG GCG TGA ACC AG-3′ |
R: 5′-CGG CGC CGG GGA GAT ACA-3′ | |
GAPDH | F: 5′-ACC ACA GTC CAT GCC ATC AC-3′ |
R: 5′-TCC ACC ACC CTG TTG CTG TA-3′ |