Figure 4

Effect of bFGF and FSH on expression of pluripotent, germ cell and PF transition specific markers by q-RTPCR: Expression of pluripotent markers (Oct-4A and Nanog), early germ cell markers (Oct-4, c-Kit and Vasa) and PF growth initiation markers (oocyte specific Gdf-9, Lhx8 and granulosa cells specific Amh) were clearly up-regulated in response to bFGF and FSH treatment compared to untreated control (except Lhx-8 in marmoset after bFGF treatment). Values depicted are mean fold change in logarithmic scale obtained with respect to untreated controls taken as 1 and 18S rRNA as house-keeping gene. Marmoset data represents results obtained from three animals, whereas human data is representative of one young and one peri-menopausal ovary. ND:Experiment not done.