Table 2 List of primer details and PCR cycling conditions used in the study
Gene | Primer sequence | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|
Pluripotent markers (Human) | |||
Oct-4A | F: AGCCCTCATTTCACCAGGCC | 57°C | 448 |
| Â | R: TGGGACTCCTCCGGGTTTTG | Â | Â |
Nanog | F: TGCAAATGTCTTCTGCTGAGAT | 57°C | 285 |
| Â | R: GTTCAGGATGTTGGAGAGTTC | Â | Â |
Early germ cell markers (Human) | |||
Oct-4 | F: GAAGGTATTCAGCCAAACGAC | 55°C | 315 |
| Â | R: GTTACAGAACCACACTCGGA | Â | Â |
Oct-4 (marmoset) | F: CCCCTGGTGCTGTGAAGCTGG | 64°C | 124 |
| Â | R: CCCCAGGGTGAGCCCCACAT | Â | Â |
c-Kit | F: CCTGGGATTTTCTCTGCGTT | 60°C | 376 |
| Â | R: ATTGGTCACTTCTGGGTCTG | Â | Â |
Vasa | F: GAC TGC GGC TTT TCT CCT ACC | 55°C | 418 |
| Â | R: TTT GGC GCT GTT CCT TTG AT | Â | Â |
PF transition markers (Human) | |||
Amh | F: CACCTGGAGGAAGTGACCTG | 60°C | 202 |
| Â | R: CCACCGCTAACACCAGGTAG | Â | Â |
Amh (marmoset) | F: ACCTGGAGGAAGTGACATGG | 64°C | 190 |
| Â | R: ACCAGGTAGTGGGTGTCTCG | Â | Â |
Gdf-9 | F: CTCCTGGAGACCAGGTAACAGGAAT | 65°C | 291 |
| Â | R: TGCACACACATTTGACAGCAGAGG | Â | Â |
Lhx8 | F: CAAGCACAATTTGCTCAGGA | 62°C | 230 |
| Â | R: GGCACGTAGGCAGAATAAGC | Â | Â |
Housekeeping gene (Human) | |||
18S | F: GGAGAGGGAGCCTGAGAAAC | 60°C | 171 |
| Â | R: CCTCCAATGGATCCTCGTTA | Â | Â |