Figure 4

Influence of PHB on FSH-induced steroid production. (A) Differentiated granulosa cells were cultured with FSH (100 ng/ml) ± T (0.5 μM) for 24 h and the levels of progesterone and estradiol in the spent medium were measured by EIA. Data are presented as mean ± SEM of four independent experiments and analyzed by two-way ANOVA and subsequently by Bonferroni post hoc test. ***, p < 0.001 (compared with LacZ). (B-C) Differentiated granulosa cells were infected with adenoviral-PHB (MOI = 40, adenoviral-LacZ as control) for 24 h and then cultured with FSH (100 ng/ml, 24 h) in the presence of T (0.5 μM). Progesterone and estradiol in the spent medium (B) were measured by EIA. Data are presented as mean ± SEM of four independent experiments and analyzed by two-way ANOVA and subsequently by Bonferroni post hoc test. **, p < 0.01; ***, p < 0.001 (compared with CTL). (C) The expression of p450scc and aromatase was examined by Western blot. Representative immunoblots from three replicate experiments are shown.