Figure 6

Down-regulation of ZEB1 reduced tumor growth and metastasis in vivo xenograft mouse. A-B. The tumor dynamic growth curves and tumor bearing mice’ survival. C. The photos of the tumor tissues dissected from the mice on day 56 after injection. D. The protein expression results from the Western blotting analysis. E-G. The protein expression results from gradation scanning analysis. H. The HE staining results from the tumor tissue sections at 400× magnification; The tumor cell metastases were visible in the samples of the CD44⁺CD117⁺WT(+++) and the CD44⁺CD117⁺ scramble (+++); a few tumor cells were seen in the lungs of the nude mice injected with the CD44⁺CD117⁺shZEB1 (+). I.The statistical analysis results of the metastatic tumor cells in the lungs of the mice injected with the different CSCs. *p < 0.05 and **p < 0.01.