Figure 4

GPER-1 specific antagonist G-15 abolished the G-1-induced inhibitory effect in ovarian cancer cells. GPER-1-positive SCOV-3 and OVCAR-3 ovarian cancer cells and GPER-1-negative HEK-293 cells were incubated for 5 days with 1 μM G-1 with or without pre-treatment with 1 μM G-15 for 24 h and the cell number was counted using MTT viability assay. Each experiment was repeated at least three times. The results are expressed as means ± SD.