Figure 2
From: Tracking NF-κB activity in tumor cells during ovarian cancer progression in a syngeneic mouse model
NF-κB reporter activity increases during ovarian cancer progression. (A) Representative BLI of WT mice injected with ID8-NGL cells or PBS (non-inject) over 90d following injection showing NF-κB reporter signal in tumor cells. Quantification of BLI in the abdominal cavity is shown in (B). BLI was significantly correlated with (C) mesenteric tumor mass and (D) volume of ascites at 90d (Spearman). (E) Luciferase activity of the NF-κB reporter was measured in harvested tumors and expressed relative to cellular protein. (F) Western blot analysis of p65 in nuclear extracts from harvested tumors. Equal loading was shown by probing for the nuclear-specific protein, histone H3. (G) Nuclear p65 expression relative to corresponding histone H3 levels was measured by densitometry. Values shown are mean + SD from 5 mice per group. ** p < 0.01 relative to 30d or 60d, Mann-Whitney test.