Figure 3
From: Tracking NF-κB activity in tumor cells during ovarian cancer progression in a syngeneic mouse model
Molecular characterization of ID8-NGL tumors. (A) H&E stain of a representative area of ID8-NGL tumors. (B) Immunohistochemical detection of the marker of high-grade serous ovarian tumors, PAX8. (C) Fluorescent microscopy showing expression of the epithelial marker pan-cytokeratin (CK) expression relative to TO-PRO-3-stained nuclei. (D) Immunohistochemical detection of the mature macrophage marker, F4/80, in tumor sections. High power (×100) confocal images of the representative tumor section showing immunofluorescent detection of (E) the proliferation marker Ki67/mib-1, (F) the GFP reporter and (G) overlap of Ki67/mib-1 and phospho-p65 (serine 276) (red) in the epithelial component of the tumor. TO-PRO-3-stained nuclei are in blue. (H) Co-expression of phospho-p65 (red) and Ki67/mib-1 (green) is observed in the nuclei of a subset of cells. The panels on the right show merged images of the corresponding boxed areas for two representative nuclei.