Figure 6
From: Tracking NF-κB activity in tumor cells during ovarian cancer progression in a syngeneic mouse model
Thymoquinone reduces NF-κB activity in tumors and expression of M2 macrophage marker in ascites fluid. (A) Effects of increasing concentrations of TQ on NF-κB reporter activity in ID8-NGL cells after 24 hours’ treatment as measured in luciferase assays. (B) Effect of 10 days treatment with 20 mg/kg TQ or PBS vehicle (thrice weekly IP injections) on NF-κB reporter activity in harvested ID8-NGL tumors. (C) QPCR analysis of the mRNA expression of the markers of M2 macrophages, mannose-receptor (mann-R) and M1 macrophages, CCL3, in RNA extracted from peritoneal lavages. Values were normalized to corresponding levels of GAPDH mRNA expression and also CK18 mRNA levels, to account for epithelial content. The expression ratio of mann-R to CCL3 is shown in (D). Values are mean + SD for 5 mice per group. * p < 0.01 relative to vehicle, Mann-Whitney test.