Figure 3

Effect of antisense IGF-1R mRNA oligonucleotides (AS) on IGF-1R expression by cancer cells. Cancer cells were arrested in serum free medium (SFM) as described in the “Methods” section for 24 hrs. Oligonucleotides at different doses were added to the medium for 48 hrs before cells were harvested for western blotting assay. (A) Western Blotting showed the cancer cells expressing more IGF-1R than the normal epithelial ovarian cells. 1 and 2 represent normal epithelial ovarian cells; 3 and 4 represent epithelial ovarian cancer cells. Actin was used for the loading control. (B) AS inhibited IGF1-R expression at different concentrations in epithelial ovarian cancer cells. Actin was used for the loading control. (C) AS also variably inhibited phosphorylated IRS-1, AKT, and MAP kinase expression in epithelial ovarian cancer cells. Actin was used for the loading control.