Figure 6
Effect of citrate on response to ABT-737 in IGROV1-R10 cell lines. Cells were treated with 10 mM citrate 24 H before exposure to 10 μM of ABT-737 and cultured for an additional 24 H (i.e. 48 H post citrate exposure). (A) Morphological features of cell layers at 72 H after citrate exposure. (B) Nuclear morphology of IGROV1-R10 cells was analysed by DAPI staining at 72 H. Bars 20 μm. (C) Cell cycle repartition was studied by flow cytometry after propidium iodide staining at 72 H after transfection in IGROV1-R10 cell lines. (For each condition, percentages of cells in the different phases of the cell cycle are indicated). (D) Protein expression levels of PARP cleavage, Mcl-1 and Bcl-xL on IGROV1-R10 cells. Actin was used as a loading control.