Figure 2

Multi- and pair-comparison statistical tests suggest moderate LSD1 mRNA overexpression in stage IIIC and grade G2/G3 specimens in our cohort. (A) Left panel: pair-comparison analysis of LSD1 mRNA levels in normal (N) and tumor (T) specimens (serous cystadenocarcinoma, TCGA cohort; log2-scale). Right panel: as in (A) but in our cohort (all tumor histological subtypes; see Additional file 3: Figure S1 for a separated analysis). (B) Left panel: multi-comparison analysis of normal tissue (N) and tumors classified by FIGO stage (I-IV). Middle and right panels: pair-comparison analyses of normal tissue (N) and stage III tumors, and stage I and stage III tumors, respectively. (C) Left panel: multiple-comparison analysis of normal tissue (N) and tumors classified as stage IIIA-IIIC. Middle and right panels: pair-comparison analyses of normal tissue (N) and stage IIIC tumors; and a pool of stage IIIA and IIIB tumors (IIIA/IIIB) and stage IIIC tumors, respectively. (D) Left panel: multiple-comparison analysis of normal tissue (N) and tumors classified by FIGO grade: borderline ( B) and G1-G3. Rest of panels: left, pair-comparison analyses of normal tissue (N) and grade G2 tumors; middle, normal tissue (N) and grade G3 tumors; and right, G2 tumors and G3 tumors. We applied the Mann–Whitney test in pair-comparison analyses, and the Kruskal-Wallis (non-parametric ANOVA) test followed by post hoc Dunn’s analysis in multiple-comparison analyses (number of comparisons = 10 in B and D, and 6 in C). P-values and number of specimens are shown on top and at the bottom of each panel. Two outliers exceeded the limits of the y-axis (values = 8.3 and 5.9) in some panels. In B-D, all histological subtypes were analyzed excluding mucinous. Whiskers in box plots represent 5–95 percentile values, and horizontal lines within boxes represent median values. P-value < 0.05 (*), p-value < 0.01 (**), p-value < 0.001 (***), and p-value < 0.0001 (****).