Table 2 Primers used for constructs and detection of the expression of miR-124.
From: MiR-124 inhibits the migration and invasion of ovarian cancer cells by targeting SphK1
Name | Sequence (5’-3’) | Tm (°C) | Amplicon (bp) |
|---|---|---|---|
Sphk1-UTR-F | ACGCTCGAGGAATTGATGGTTAGCGAGGCC | 64.5 | 1155 |
Sphk1-UTR-R | AGGGCGGCCGCTTATTTGGATTTGGTTCGTGGG | 65 | |
Sphk1-UTR-Mut-site-F | GACCCCTGGGCCGCGCTGTCCGTAAGTGTCTACTTGCAGGACC | 85 | 6658 |
Sphk1-UTR-Mut-site-R | GGTCCTGCAAGTAGACACTTACGGACAGCGCGGCCCAGGGGTC | 84 | |
U6-F | CGCTTCGGCAGCACATATAC | 59.4 | 64 |
U6-R | CAGGGGCCATGCTAATCTT | 57.5 | |
MiR-124-F | GATACTCATAAGGCACGCGG | 60.6 | 60 |
MiR-124-R | GTGCAGGGTCCGAGGT | 57.9 | |
MiR-124-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGCATTCT | 87.8 |