AGTR1 expression patterns in ovarian cancer with hypermethylated promoter-mediated BRCA1 dysfunction . Ai, location of CpG sites in the core promoter region of BRCA1. Genomic coordinates are shown, along with the primer-amplified fragments, GC percentage, location of individual CpG dinucleotides (dashes) and BRCA1 RefSeq gene (exon 1 shown as a blue box and intron shown as an arrowed line). The arrow indicates the direction of transcription. Aii and Aiii, comparative analysis of methylation patterns in the core promoter region of BRCA1 and their adjacent normal tissue (each group, n = 15). The circles correspond to the CpG sites denoted by black dashes in Figure
3Ai. Closed circles, methylation; open circles, unmethylated. Ten individual clones were sequenced for each sample. Bi and Ci, summary of the methylation levels of BRCA1 core promoter from the measurements shown in Aii and Aiii, respectively. Bii and Cii, relative BRCA1 mRNA levels were measured in ovarian cancer with identified hypermethylated or unmethylated BRCA1 promoter compared to their adjacent normal tissue, respectively (each group, n = 15). Biii and Ciii, relative AGTR1 mRNA levels were measured in ovarian cancer in the presence or absence of BRCA1 dysfunction, respectively (each group, n = 15). Bar graphs show mean ± SD. *P < 0.05 vs. Normal.