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Figure 2 | Journal of Ovarian Research

Figure 2

From: Sequence variation among members of the miR-200 microRNA family is correlated with variation in the ability to induce hallmarks of mesenchymal-epithelial transition in ovarian cancer cells

Figure 2

Over expression of the miR-200 family induces morphological changes consistent with the induction of MET. (A) Multiple sequence alignment of the miR-200 family. Alignment is performed using the ClustalW2 program. Sequence differences relative to miR-429 are highlighted. (B) Representative phase contrast microscopy images of (i) HEY cells (not transfected), HEY cells transfected with (ii) nc-miR, (iii) miR-200a, (iv) miR-141, (v) miR-200b, (vi) miR-200c, and (vii) miR-429, 48 hours post transfection. Scale bars, 50 μm. (C) The accumulation of rounded epithelial-like cells occurred in miR-200 family transfected cells. Values represent mean ± SEM (n = 124-211 cells analyzed per group). Asterisks represent significant differences from the negative control (nc-miR) (*P <0.0001, NS = not significant, Mann-Whitney U test).

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Journal of Ovarian Research

ISSN: 1757-2215

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