Figure 1
The inhibitory effect of MOC31PE immunotoxin on protein synthesis and cell viability. B76 cells were seeded in 48-well plates (A) and the next day the medium were changed and added IT and/or CsA. Protein synthesis was analysed after 24 h, measuring the amount of [3H]-leucine protein incorporation. Values for treated cells are shown as the percentage of the values obtained in non-treated control cells. One representative experiment with three wells (average ± SD) for each treatment is shown. The experiment was repeated twice. For the cell viability test (B), cells were seeded in 96-well plates and added IT and/or CsA as described above. The MTS reagent was added after 24 h treatment and absorbance read after additional 4 h. Average ± SD of three independent experiments is shown. In each experiment each treatment were tested at least in triplicate wells. The lower graph (B) is one representative experiment of three independent experiments with treatment for 48 h. Average ± SD for each treatment tested in triplicate is shown.