Characterization of ovarian stem cells on Day 0 and Day 7 by confocal imaging. Pluripotent markers (A) OCT-4 (B) SSEA-4 (C) CD133; PGC markers (D) FRAGILIS (E) STELLA and germ cell markers (F) DAZL (G) VASA were immuno-localized to the VSELs and OGSCs at Day0. The oocyte-like structures observed on Day7 post culture expressed germ cell markers (H) DAZL, (I) GDF-9 (J) VASA (K) SCP-3 and (L) OCT-4. DAPI is used as a nuclear counter stain. (M-O) Mouse, rabbit and goat negative controls with omission of primary antibody were immuno-stained similarly. Scale bar = 5 μm. Merged image of DAPI, FITC and DIC (Differential Interference contrast).